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0The paper introduces EnrichSci, a sophisticated new method for single-nucleus RNA sequencing that specializes in profiling rare cell types. Unlike traditional techniques that rely on expensive antibodies or restrictive microfluidics, this platform uses RNA fluorescence in situ hybridization to identify and isolate specific cells from complex tissues. By applying this approach to oligodendrocytes in the aging mouse brain, researchers successfully identified molecular shifts and exon-level changes that are often missed by standard analysis. The workflow is designed to be highly scalable and cost-effective, allowing for deep sequencing of genomic elements across millions of cells. Ultimately, the sources demonstrate how EnrichSci provides a high-resolution view of cellular aging and regulation within diverse biological samples.
References:
Liao A, Zhang Z, Sziraki A, et al. Transcript-guided targeted cell enrichment for scalable single-nucleus RNA sequencing[J]. Cell Genomics, 2026, 6(3).
