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0The paper introduce RT&T-AMP-MERFISH, a cutting-edge spatial transcriptomics technique that enables isoform-resolved imaging across the entire transcriptome. By combining in situ RNA amplification with multiplexed error-robust fluorescence in situ hybridization, researchers can now detect approximately 33,000 distinct RNAs at single-cell and subcellular resolution. This methodology was successfully applied to mouse brain tissues, creating a high-resolution atlas that identifies complex gene programs and cell-cell communication networks. The data specifically highlight how transcript isoform usage varies significantly across different anatomical regions and specific cell types. Ultimately, this resource provides a scalable framework for exploring the molecular and cellular architecture of intact tissues.
References:
Cohen L, Halpern A R, Blosser T R, et al. Whole-transcriptome-scale isoform-resolved spatial imaging of single cells in tissues[J]. Cell, 2026.
