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0The research introducing RT&T-AMP-MERFISH describes a breakthrough in spatial transcriptomics by enabling the simultaneous imaging of approximately 33,000 distinct RNAs at single-cell resolution. This method utilizes an in situ RNA amplification technique to overcome the limitations of traditional imaging, allowing for the detection of short sequences and isoform-specific variations across the mouse brain. By mapping these transcripts, researchers identified complex gene modules and cell-cell communication patterns that correspond to specific anatomical regions. The data reveals how different transcript isoforms are used uniquely by various cell types, offering a more detailed understanding of molecular architecture than previously possible. Ultimately, this technology provides a scalable platform for exploring the cellular basis of tissue function and post-transcriptional regulation in intact biological samples.
References:
Cohen L, Halpern A R, Blosser T R, et al. Whole-transcriptome-scale isoform-resolved spatial imaging of single cells in tissues[J]. Cell, 2026.
