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0The paper introduces WHOLISTIC, a novel whole-organism live-imaging framework designed to simultaneously record second-timescale cellular activity across all tissues in transparent vertebrates like larval zebrafish. By integrating volumetric fluorescence microscopy, pancellular calcium indicators, and advanced motion-correction algorithms, this system overcomes previous optical barriers to map dynamic intercellular interactions body-wide. The platform successfully identifies functional tissue ensembles, uncovers novel muscle synergies and nerve innervations, and tracks inter-organ coupling such as brain-body interactions and ependymal cell responses. Furthermore, WHOLISTIC facilitates the discovery of previously uncharacterized ultraslow oscillations during motor quiescence, establishing a powerful paradigm for systems biology.
References:
Ruetten V M S, Zheng W, Siwanowicz I, et al. Imaging cellular activity across all organs reveals body-wide circuits[J]. Nature, 2026: 1-11.
